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Fig. 10
Fin development in morpholinoinhibited buds and in hst heterozygous adults. (A-D) Methylene Blue/Azure II-stained transverse sections of pectoral fins at 6 dpf. (A) Wild-type pectoral fin, showing normal development of ventral and dorsal musculature and the endoskeletal disc. (B-D) Pectoral fins of embryos injected with 1.7 ng tbx5-MO show a range of defects, including shortened proximal-distal length and up-turned orientation. (B) Frequently, muscle and endoskeletal disc development appears normal proximally, but is poorly developed in the distal half of the fin. (C) Other fins show greatly thickened endoskeletal disc development or (D) severely stunted growth. (E,F) Pectoral fin development in hst heterozygous adults. (E,F) Alcian Blue/Alizarin Red preparations of adult pectoral fins. Lepidotrichia growth and branching are comparable in (E) wild-type and (F) hst heterozygous adults. Fin shapes and sizes are similar. dm, dorsal musculature; ed, endoskeletal disc; vm, ventral musculature.