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Fig. S16
3D imaging ability comparison of regular SPIM and TLS-SPIM on a nucleus-labeled zebrafish embryo. (a, b) XY and XZ orthogonal slices of a ~15 hpf zebrafish embryo tailbud imaged by TLS-SPIM (NAOD=0.2, NAID=0.05, single excitation beam, nine tiling positions, raw image). (c, d) XY and XZ orthogonal slices of the same embryo imaged by regular SPIM with a Gaussian light sheet (NAOD=0.07, single excitation beam, raw image). (e, f) Magnified views of the selected areas in a and c. (g, h) Magnified views of the selected area in b and d. Scale bars, 20 µm (a-d), 5 µm (e-h).
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