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Fig. S4 MCC counting method. (A) Representative images of odf3b expression in the zebrafish pronephros at 24 hpf of traditional WISH (purple) and fluorescent WISH (red). Black and white arrowheads denote individual odf3b+ cells. (B) Quantification of average MCC number as marked by odf3b comparing control and etv5a MO embryos analyzed by WISH (n=33 each) and fluorescent WISH (n=10 each). There is no significant difference between the two controls or the two groups of etv5a morphants determined by two-tailed student’s t-test. P-value *****p<0.001, N.S. (non-significant).
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Reprinted from Developmental Biology, 411(2), Marra, A.N., Wingert, R.A., Epithelial cell fate in the nephron tubule is mediated by the ETS transcription factors etv5a and etv4 during zebrafish kidney development, 231-45, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.