ZFIN Image: Shiu et al., 2016, Fig. 4

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Figure Caption

Fig. 4

Ubiquitous YY1a inhibition by injection of morpholinos (YY1-MO). a Western-blot analysis of YY1a proteins in the 24-h stage wild type embryos and YY1-MO-injected embryos. The knockdown of YY1a gene in these embryos by YY1-MO injection was shown in panel a, lane 3 (panel a, lanes 1-3; panel b, the positive control, HeLa cell lysate). Actin was the loading control. b Each one-cell stage embryo was injected with either 25 ng of control-MO or 25 ng of YY1-MO. At 12, 24, and 48 hpf, embryos were fixed and examined after in-situ hybridization. The phase-contrast images of the wild type (panels, a, d, and g) and control-MO-injected (panels b, e, and h) embryos show normal development and YY1a expression patterns. However those of the YY1-MO-injected embryos show abnormal development (panels c, f and i) and delayed YY1a expression (panel c, 12 hpf; arrows) and abnormal YY1a expression (panel f and i; 24 and 48 hpf, respectively; arrows). c Phase-contrast images of wild type embryos (panel a), control-MO-injected embryos (panel b), YY1-MO-injected embryos that are slightly affected (panel c), and YY1-MO injected embryos that are severely affected (panel c). The abnormal brain and heart are indicated by arrows. Bars indicate 250 µm

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biomed. Sci.