ZFIN Image: Vaithianathan et al., 2016, Fig. 1 S1

Image

Figure Caption

Fig. 1 S1

Overview of the experimental procedure.(A) Retinas were removed from transgenic zebrafish that expressed Vglut1-paRFP fusion protein after heat shock. (B) Intact, acutely dissociated retinal BPCs were voltage-clamped via a whole-cell patch pipette placed on the synaptic terminal. (C) Electron micrograph showing the synaptic terminal of a zebrafish BPC, with the boxed region around a ribbon at higher magnification in the inset. Ribbons are surrounded by tethered synaptic vesicles. (D) Cartoon illustrating photoactivation of paRFP by 405-nm light. (E) Example of red fluorescence in the synaptic terminal before and after photoactivation.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife