ZFIN Image: Jaffe et al., 2016, Fig. S2

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Figure Caption

Fig. S2

Related to Figure 1. kur Alleles Carry c21orf59 Mutations.(A) Schematic diagram of the region kur was mapped to, including c21orf59. (B) Western blot of Myc-tagged Kur after expression in zebrafish embryos which were subsequently raised at the given temperatures. At higher temperatures, wild-type Kur is present as a strong band but Kur^tm304 protein has either not been generated or has decayed. Gamma-tubulin was used as a loading control. (C) kur mutants and morphants were rescued (body axis defect was completely rescued, whereas kidney cysts (white arrows) were partially rescued) by injection at the 1-cell stage of either zebrafish or human c21orf59 RNA. (D) Heart looping scored by myl7 ISH at 48 hpf; mutants and morphants exhibited laterality defects that were not present in uninjected or sibling controls. Statistical comparison of different genotypes was performed using chi-square analysis with P < 0.05 being considered to report significant differences.

Acknowledgments

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