IMAGE

Fig. S7

ID
ZDB-IMAGE-160405-10
Source
Figures for Webb et al., 2016
Image
Figure Caption

Fig. S7

Expression of tailbud markers in vivo and in low-density cultures of segmentation clock cells.(A) z-stack projection showing the expression patterns of Ntla (green) and Tbx16 (red) protein in a 12-somite stage wild type embryo detected using immunohistochemistry with monoclonal antibodies D18-4 (IgG1) to Ntla and C24-1 (IgG2a) to Tbx16. Scale bar = 120 µm (B-C) Close up view of the boxed area in (A) showing a single confocal section at dorsal (B) and ventral (C) locations in the tailbud. Scale bar = 60 µm. (D) Representative panels showing expression of Ntla (green) and Tbx16 (red) in single cells within low-density tailbud cultures (serum + Fgf8b) after 5 hr in vitro. Nuclei are labeled with DAPI (blue). Cells single-positive for Ntla and Tbx16 are visible, as are cells co-expressing both proteins. Scale bar = 20 µm. (E) Quantification of nuclear fluorescence intensity of experiment in (D) showing populations of Ntla-positive, Tbx16-positive, and Ntla/Tbx16 co-expressing cells.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife