ZFIN Image: Marelli et al., 2016, Fig. 3

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Figure Caption

Fig. 3

Panel A) Morphological evaluation of phenotypes of embryos injected with thraa or thrb splicing morpholinos (thraa_MOs or thrb_MOs) and comparison with the mismatch controls (mism_ctrls) at 24 hpf. Thraa_MOs: G1, normal/mild phenotype, similar to the mism_ctrls; G2, moderate phenotype (small head, reduced body length and hypopigmentation); G3: severe phenotype. Thrb_MOs: G1, normal/mild phenotype, similar to the mism_ctrls; G2, moderate phenotype (curved/curled tail, notochord abnormalities and hypopigmented eyes); G3: severe phenotype. The number of embryos in each phenotypic group is reported in parenthesis. Images are representative of 3 independent experiments. Panel B) qRT-PCR of wild-type and deleted thraa and thrb mRNA expression after splMOs injection using specific sets of primers (see Materials and Methods for details). ***p < 0.001 vs wild-type zTRs. Panel C) Analysis of lethality during development (from 1.5 to 10 dpf) of thraa_ and thrb_MOs and mism_ctrls. The results are expressed as percentage of 3 independent experiments (100 embryos each).

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Phenotype details

Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 424, Marelli, F., Carra, S., Agostini, M., Cotelli, F., Peeters, R., Chatterjee, K., Persani, L., Patterns of thyroid hormone receptor expression in zebrafish and generation of a novel model of resistance to thyroid hormone action, 102-17, Copyright (2016) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.