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Fig. S2
Conditional activation/repression of Mnx1 target genes. Scheme of constructs used for the generation of mnx1 inducible transgenic fish lines (A). These transgenic lines contain either mnx1 in its transcriptional repressor form or a VP16-coupled transactivativing form of mnx1. Expression of the Mnx1 transcriptional repressor/activator is induced by the heat-shock promoter hsp70. The co-activation of a fluorescent YFP protein enables verification of efficient transgene activation (B, B′′, D, D′′). Activation of mnx1 was further confirmed through increased mnx1 expression in ISH stainings (C, E).
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Reprinted from Developmental Biology, 411(1), Ott, E., Wendik, B., Srivastava, M., Pacho, F., Töchterle, S., Salvenmoser, W., Meyer, D., Pronephric tubule morphogenesis in zebrafish depends on Mnx mediated repression of irx1b within the intermediate mesoderm, 101-14, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.