Fig. 7

Fig. 7

Knockdown of irx1b in mnx morphants partially rescues pronephric phenotypes. Shown is a lateral view of Acetylated tubulin stained Tg(cldnb:lynEGFP) embryos that were injected with Mo-mnx alone (A, A′) or in combination with Mo-irx1b (B, B′). Measurement of tubule widths shows partial rescue of the MO-mnx phenotype in the combination mnx/irx1b double knockdown (C). Excretory defects in Mo-mnx injected embryos are rescued by additional irx1b knockdown (D). TEM sections of embryos co-injected for mnx and irx1b morpholinos demonstrate reduced pronephric tubule width and restored morphology of microvilli (E, E′; Scale bars correspond to 5 and 2 µm, respectively).