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Fig. 3
BODIPY-Labeled Lipid Injections Were Incorporated into New Lipids in the Yolk Sac
(A, B, D, and E) BODIPY-labeled FFA and PC were injected into yolk sac of the embryos at 24 hpf. Fluorescent imaging shows spreading of fluorescent FFA (A) and PC (D) at 1 hpi (as marked by dotted white lines) that continued to spread throughout the yolk sac at 6 hpi (B and E).
(C) TLC of BODIPY-labeled FFA injections, with lipid standards in pale orange, shows fluorescent bands in yolk sac samples at 1, 3, and 6 hpi (white arrowheads) and a band at 6 hpi in the body sample (asterisk) that correspond to the TG standard. Bands below the TG bands are present in yolk sac samples at 1, 3, and 6 hpi (open arrowheads) while a similar band is only detected at 6 hpi in the body sample (pound sign). Groupings of four bands, just above the cholesterol standard, were present in yolk samples at 1 hpi and 3 hpi (brackets). One band only was detected in the adjacent area in body samples at 1 hpi and 3 hpi (cross). At 6 hpi, another band is detected above these bands in both the yolk sac and body samples that were not present at earlier time points (circle) demonstrating a temporal dynamic evolution of lipid during development.
(F) TLC of BODIPY-labeled PC injections produced a pair of bands between the TG and FFA standards only in the yolk sac samples at 1, 3, and 6 hpi (arrows, parentheses). These bands were never detected in the body up to 6 hpi. Bands that were located at a level consistent with the DG standard were detected in yolk sac samples at 1, 3, and 6 hpi (red arrowheads) but were never observed in the body up to 6 hpi.
See also Figure S3.