ZFIN Image: Kawasaki et al., 2016, Fig. 3

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Figure Caption

Fig. 3

Propagation of SSCs in culture. (A,B) Effects of basal medium (A) and heparin at 25-50U/ml (B) on proliferation of early spermatogonia. Grafts of the sox17::egfp testicular hyperplasias recovered from rag1^t26683 mutant host were dissociated and cultured under various conditions. The gas condition was adjusted to the basal culture medium; in air for L-15 and in 5% CO₂ for DMEM. The culture medium in B was DMEM with 5% CO₂ and 20% O₂. Bars indicate the mean (n=4). (C) Dissociated sox17::egfp testicular hyperplasias were cultured for 1, 31 and 48days under SSC propagation conditions. Inset shows the morphology of the early stage of spermatogonia. Top panels: phase contrast; bottom panels: fluorescence. Arrows indicate GFP-negative differentiated spermatogonia. Scale bar: 50µm.

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