ZFIN Image: Eom et al., 2015, Fig. 5 S3

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Figure Caption

Fig. 5 S3

Notch signaling inhibition.(A) Treatment with Notch inhibitor LY411575 during adult pigment pattern development caused defects in melanophore clearance and stripe consolidation. (B) Induction of mitfa:Tet:nVenus-2a-dnSu(H) in melanophores of stable transgenics reduced expression of Notch target gene her6 (p<0.05) in isolated melanophores, relative to non-transgenic but dd-treated controls. (C) Melanophore of fish mosaically expressing mitfa:Tet:nVenus-2a-dnSu(H) stained for nuclear-localizing Venus by immunohistochemistry (arrow); nucleus is bipartite. Scale bar. (A) Extended induction of mitfa:Tet:nVenus-2a-dnSu(H) in melanophores did not affect total numbers of xanthophores (t₈=0.92, p=0.4) or melanophores (t₈=0.70, p=0.5) relative to dd-treated non-transgenic controls. Fish analyzed here were performed concurrently with those of Figure 4 - figure supplement 2E and employed the same controls. Scale bars: 200 µm (A); 20 µm (C).

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