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Fig. 3
The hyperbranching vasculature phenotype, observed in Pten double homozygous zebrafish embryos at 3dpf, can be rescued by wild type Pten.
Zebrafish embryos from a Tg(kdrl:eGFP) ptena+/-ptenb-/- incross were microinjected at the one-cell stage with 300 pg synthetic mRNA encoding Ptenb-mCherry. At 3dpf the embryos were analyzed for the hyperbranching vessel phenotype by confocal live imaging on a Leica TCS-SPE microscope (anterior to the left, 20x objective, 2µm z-stacks). Subsequently, the embryos were genotyped. Pictures show the trunk region distal from the urogenital opening of representative embryos; non-injected control embryos (control) were included for reference.