|
Fig. 6
DHS inhibits metastasis of LLC cells in a dose-dependent manner.
(a) Fluorescent micrographs of DiI-labeled LLC cells (red) and host endothelial cells (green) in 5 days old fli1:EGFP transgenic zebrafish embryos, 3 days following tumour cell implantation and treatment with vehicle, DHS at 0.01 µM, DHS at 0.1 µM or DHS at 1 µM. The tumour and main metastatic site in the caudal venous plexus are indicated by white boxes and shown in higher magnification below. White arrows indicate metastasized tumour cells. (b) Quantification of the number of metastatic foci in the caudal venous plexus, 3 days after LLC implantation. *p ≤ 0.05, **p ≤ 0.01. n = 20, 15, 22 and 15 for vehicle-treated, DHS 1 µM, DHS 0.1 µM and DHS 0.01 µM groups respectively.