Fig. S4
Micrometastasis formation occurs in cxcl12 single ligand mutants. Engraftment of MDA-MB-231-B cell line resulted in micrometastasis formation in the tail fin region, characterized by invading single cells in both the cxcl12b -/- mutant and wt siblings at 2 and 4 dpi (A) and tumor burden in wt, cxcl12a -/- and cxcl12b -/- larvae at 2 dpi (B and C). The trend was not statistically significant (One-way ANOVA and Bonferroni post hoc test). Number of larvae in (A): n=30 (wt 2 dpi), n=20 (cxcl12b -/- 2 dpi), n=19 (wt 4 dpi) and n=15 (cxcl12b -/- 4 dpi). Number of larvae in (B): n= 23 (wt), n=27 (cxcl12a -/-) and n=27 (cxcl12b -/-). Scale bars: 50 µm.