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Fig. S5
Gal4/UAS-based bidirectional expression system.
(A) The constructs of the Gal4/UAS-based bidirectional expression system. Heat shock (HS) treatment activates the hsp70I promoter in the driver construct (Driver) to express Gal4-VP16. Gal4-VP16 protein binds to the UAS on the responder construct (Responder) and activates the expression of mCherry and EGFP via E1b minimal promoters. (B) A positive correlation of expression level between the two genes flanking the UAS in Tg(hsp:Gal4-VP: EGFP:UAS:mcherry). The scale bar represents 1 mm.
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