ZFIN Image: Guo et al., 2016, Fig. 6

Image

Figure Caption

Fig. 6

Downregulation of wnt5b during gastrulation by lbx1b overexpression.

(A) In situ hybridization for non-canonical Wnt/PCP ligands (wnt5b and wnt11) in control and lbx1b-overexpressing embryos at 90% epiboly. Dorsal views (anterior to the top) for wnt5b and wnt11 of embryos injected with buffer (ctrl) or lbx1b mRNA (lbx1b). (B) Incidence of the defective expression patterns observed in the embryos shown in panel A. Significant differences (*p < 0.01) were observed for wnt5b. The numbers of control and lbx1b zebrafish embryos were 22 and 21 for wnt5b and 23 and 22 for wnt11, respectively. NS: not significant. (C) Decreased wnt5b expression in 90% epiboly embryos injected with lbx1b mRNA by quantitative RT-PCR assays. *p < 0.01. (D) In vivo luciferase assay in 90% epiboly embryos injected with control vector (luc) or the putative promoter regions (P1 and P2) of zebrafish wnt5b. P2 showed higher transcriptional activity than P1. Co-injection of lbx1b mRNA in P2 significantly repressed the transcriptional activity. *p < 0.01. AU, arbitrary unit.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.