ZFIN Image: McPherson et al., 2016, Fig. 2

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Figure Caption

Fig. 2

th2⁺ Cells Are Continuously Generated throughout Life

(A) 5 dpf larvae were treated with BrdU for 24 hr and analyzed at 9 dpf. Numerous th2:GFP⁺ cells (green) are labeled with BrdU (magenta) in the hypothalamic posterior recess. Arrowheads indicate double-labeled cells.

(B) 12 dpf larvae were treated with BrdU for 24 hr and analyzed at 25 dpf. th2:GFP⁺ cells (green) labeled with BrdU (magenta) can be found in medial regions of the hypothalamic posterior recess. Arrowheads indicate double-labeled cells.

(C) 6 mpf fish were injected interperitoneally with BrdU and analyzed 2 weeks later. th2:GFP⁺ cells (green) labeled with BrdU (magenta) can be found in a midsagittal view of the hypothalamic posterior recess. Arrowheads indicate double-labeled cells.

(D) Percent of BrdU⁺ cells in the th2:GFP⁺ population throughout the entire posterior recess. Error bars represent SEM; n = 5 brains.

Images in (A) and (B) are ventral maximum intensity confocal z projections of the hypothalamic posterior recess. Images in (C) are maximum intensity confocal z projections from midsagittal views of the hypothalamic posterior recess. Individual and merged channels of boxed region are shown in the lower panels. The scale bars represent 10 µM.

Acknowledgments

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