Fig. S12
Insulin knockdown has no effect on δ cell number or total endocrine cell number. (A,B) Confocal projections of 3 dpf control (A) and insaMO-injected (B) islets immunostained for Insulin (green), Somatostatin (red), and DNA (blue). (C) Quantification of somatostatin-positive cells in control (n=5) and insMO-injected (n=6) embryos at 3 dpf. No significant change was evident in δ cell mass. (D) Quantification of endocrine cells in control and insaMO-injected embryos from 1 dpf to 4 dpf. n≥7 for all time points. (E-L) Confocal projections of control (E-H) and insaMO (I-L) islets in doubly transgenic Tg(neurod:GFP); Tg(ins:dsRed)embryos. Student’s t-test was used in C and two way ANOVA was used in D to determine significance.
Reprinted from Developmental Biology, 409(2), Ye, L., Robertson, M.A., Mastracci, T.L., Anderson, R.M., An insulin signaling feedback loop regulates pancreas progenitor cell differentiation during islet development and regeneration, 354-69, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.