ZFIN Image: Ye et al., 2016, Fig. 7

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Figure Caption

Fig. 7

Insulin signaling blockade in transplanted endodermal committed blastomeres promotes Pdx1 expression. (A) Scheme of endoderm replacement transplantation experiments. sox32-injected blastomeres termed “Endoderm Committed Blastomeres” (ECBs) were isolated from donor Tg(sox17:GFP) blastulae with or without injected dnIRS2-GFP mRNA. ECBs were transplanted into endoderm deficient, sox32MO-injected host blastulae. (B) 24 hpf chimera shows extensive replacement of endogenous endoderm with transplanted Tg(sox17:GFP) donor cells. (C–D′′) Merged and single channel confocal projections of 24 hpf embryos with endoderm transplants from sox32 mRNA (C–C3, n=8) or sox32 and dnIRS2 mRNA (D–D′′, n=7) injected donors. Embryos were immunostained for GFP (green), Pdx1 (red), and DNA (blue). dnIRS2-GFP-expressing endoderm showed increased Pdx1 expression.

Acknowledgments

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Reprinted from Developmental Biology, 409(2), Ye, L., Robertson, M.A., Mastracci, T.L., Anderson, R.M., An insulin signaling feedback loop regulates pancreas progenitor cell differentiation during islet development and regeneration, 354-69, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.