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Fig. 1
Establishment of a zebrafish model to quantify mouse melanoma cell behaviors in vivo.(a) Schematic of experimental protocols. (b) Left panels: Representative images show that 500 tfRFP B16 cells were injected into the yolk sac (white arrow) of a zebrafish. Right panels: the fish was divided into three main sections (Head, Trunk and Tail) to quantitate metastatic sites of the tumor cells. Scale bar, 500 µm. (c) Approximately five hundred tfRFP B16 cells were injected into the yolk sac of 2 day post-fertilization (2 dpf) embryos. Left, middle, and right panels: fluorescent images of the fish at 0, 3, and 6 dpi. Images are representative of >100 injected embryos. Arrowheads indicate disseminated tumor foci (single tumor cells or cell aggregates). Color code: in (a), embryos are yellow, mouse melanoma cells are red; in (c), zebrafish blood vessels are green and melanoma cells are red. Scale bar, 500 µm. Inset: yellow-dashed line rectangle box is the enlarged image of the small yellow box above; scale bar, 100 µm.