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Fig. 4
Flp-mediated inversion and excision of SAGFLEx. (A) Schematic representation of the events of SAGFLEx integrated into intron 2 of the lima1a locus in Tg(FLEx-lima1a:GFP) after Flp mRNA injection. The position of the primers used for the allele-specific PCR (E2-for/pA-for/GFP-rev) are indicated. (B) Native GFP fluorescence in uninjected and Flp mRNA injected Tg(FLEx-lima1a:GFP) embryos. Lateral and dorsal views at 28 hpf. (C) Allele-specific PCR of uninjected and Flp mRNA injected Tg(FLEx-lima1a:GFP) embryos with the primers indicated above.
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