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Fig. 9
EGFP expression in transgenic embryos with the mutant 316-bp promoter constructs. Fertilized one-cell eggs were injected with the 316-bp promoter constructs having point mutations in the indicated GATA and A/T-rich elements. Typical images of the trunk region of the 2-dpf (A–F) or 3-dpf (G and H) embryos are shown. The arrowheads indicate EGFP signals in the trunk muscle. The asterisks indicate yolk sac autofluorescence. Bars, 200 µm.
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Reprinted from Gene, 577(2), Miyagi, H., Nag, K., Sultana, N., Munakata, K., Hirose, S., Nakamura, N., Characterization of the zebrafish cx36.7 gene promoter: Its regulation of cardiac-specific expression and skeletal muscle-specific repression, 265-74, Copyright (2016) with permission from Elsevier. Full text @ Gene