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Fig. 5
Cdx4 regulates the establishment phase of hoxc genes in the neuroectoderm but not in the paraxial mesoderm. A–X: Expression analysis of trunk hoxc genes in wild-type (A–L) and Cdx4-deficient (M–X) embryos during early (6 somite/12 hpf; A–D, M–P), mid (12 somite/15 hpf; E–H, Q–T) and late (18 somite/18 hpf; I–L, U–X) segmentation stages. A,E,I,M,Q,U: hoxc6a. B,F,J,N,R,V: hoxc8a. C,G,K,O,S,W: hoxc10a. D,H,L,P,T,X: hoxc6b. Expression of hox genes (purple) was aligned using krx20 (rhombomere 3 and 5; red) as a landmark. Anterior limit of hox expression in in the neuroectoderm is indicated with an arrow, and in the paraxial mesoderm with an arrowhead. Embryos were flat mounted anterior to the left. Scale bar = 200 microns.