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Fig. 9 S4
The expression of neither vegfr2 nor kdr is not altered in the trunk vessels of dll4 morphants.
Whole-mount in situ hybridization (WISH) analyses of the embryos (26–27 ss) uninjected or injected with dll4 MO using antisense probe for vegfr2 (also termed kdrl) and kdr (kdrb), a paralog of vegfr2. Note that the expression of vegfr2 and kdr mRNA in the DA and the sprouting ISVs observed in wild-type embryos is not altered in dll4 morphants. A set of representative images of two independent experiments is shown. Scale bar, 100 µm. DA, dorsal aorta.
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