ZFIN Image: Yokota et al., 2015, Fig. 11

Image

Figure Caption

Fig. 11

Dll4 does not regulate Ca²⁺ oscillations in stalk cells after they completely migrate out of the DA.

(A) 3D-rendered time-sequential images of Tg(fli1:Gal4FF);(UAS:GCaMP7a);(fli1:H2B-mC) embryos after stalk cell budding from the DA injected with dll4 MO (27 ss). Green and orange arrowheads indicate tip and stalk cells, respectively. (B) The fluorescence changes in GCaMP7a (ΔF/F₀) of individual ECs from A indicated by arrowheads at the left panel are shown as a graph. (C) Ca²⁺ oscillation frequency (left) and mean ΔF/F₀ (right) in tip cells, stalk cells, and other ECs within the DA in control MO- or dll4 MO-injected embryos after stalk cell budding from the DA as illustrated at the left panel. (n ≥ 9). Scale bar, 10 mm in A.

Figure Data

Expression / Labeling details

Acknowledgments

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