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Fig. 3
Inhibitory effects of F56 on angiogenesis of the CAM and zebrafish embryo. A. Chicken eggs (n = 5 per group) were incubated for 3 days and then opened with electric drill, whole egg contents were removed into Petri dishes. The autoclaved filter papers were used as carriers for peptides and VEGF applied to the CAM. After cultured for 48 hr, areas around the filter papers were photographed and the number of large vessels, small vessels and capillaries were counted. B. Embryos with suitable developmental stages were selected, then drugs were injected into the yolk sac of 2 dpf (2 days after fertilization) zebrafish. There were 30 zebrafish treated each experimental group. When incubated to 3 dpf, 10 zebrafish of each group were randomly selected, fluorescence of the SIVs (subintestinal vein vessels) was detected and photographed. Image analysis was performed by calculating the SIVs area. Data are expressed as mean + SEM. *P < 0.05; **P < 0.01; ***P < 0.001.