ZFIN Image: Beerman et al., 2015, Fig. S2

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Figure Caption

Fig. S2

(Relates to Figure 1). Detailed analysis of calcium activity profile from a migrating neutrophil.

The top image of a cell illustrates how the boxes L=4, 20, 36, 52, 68, 84, and 100 from Figure S1 were rotated, stretched, and magnified to highlight the enriched calcium flux at the leading edge, and to a lesser extent the lagging edge over time. The y axis is time (min) and the x axis is the GCaMP3/dsRed ratio oriented such that the turquoise line shows a visual threshold of the ratio at 15 and the dark-blue line shows a visual threshold of the ratio at 10. The time-lapse ratiometric cell images on the left correspond approximately to the time axis on the calcium activity profiles to provide a visual readout of the quantified signal graphed adjacently.

Acknowledgments

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