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Fig. 5
Comparing Resolution of LFM and SPED Light Sheet Methods
Three-dimensional volumes were acquired from a 10 dpf Tg(elavl3:H2B-GCaMP6s) zebrafish larva with LFM and SPED light sheet microscopy, using 10×/0.6NA (water immersion, Olympus) objective with 500 ms exposure and 10×/0.3NA (air, Olympus) objective with 460 ms exposure, respectively. SPED light sheet images in Figure 6B were acquired with less than 100 ms exposure/volume, still yielding cellular resolution. Scale bars, 100 µm.
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Reprinted from Cell, 163, Tomer, R., Lovett-Barron, M., Kauvar, I., Andalman, A., Burns, V.M., Sankaran, S., Grosenick, L., Broxton, M., Yang, S., Deisseroth, K., SPED Light Sheet Microscopy: Fast Mapping of Biological System Structure and Function, 1796-806, Copyright (2015) with permission from Elsevier. Full text @ Cell