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Fig. S5
Tissue morphology of gut (A), kidney (B) and liver (C) in sterol 6 dpf zebrafish larvae. Six dpf larvae was left untreated (Control), enriched in 7-ketocholesterol (7KC, 100 µm, 30 min) or depleted of cholesterol with methyl-β-cyclodextrin (mβCD, 2.5 mm, 40 min) as in Figure 3, fixed and stained with antibodies against the tight junction marker ZO-1 (red), WGA (yellow) to mark the cell surface, CTxB (green) to label GM1-rich membranes and DAPI (blue) to visualize cell nuclei. Scale bar in merged images is 20 µm; scale bar in zoomed merged images is 5 µm.
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