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Fig. S4
Generation and validation of a 12xNRE:EGFP Notch signaling reporter (A) Schematic illustration of the 12xNRE Notch reporter, consisting of 12 Notch-responsive elements (12xNRE) and a rabbit β-globin minimal promoter (MP), followed by enhanced GFP (eGFP) and polyA (pA) tail, flanked by Tol2 elements. (B,C) Dose-response of Notch signaling in 12xNRE:EGFP embryos, following treatment with the Notch signaling inhibitor DAPT. (D,E) Quantification of EGFP intensity following treatments in B and C. Results are shown in arbitrary fluorescence units (AFU). n=6 per treatment. ***P<0.0001. (F,G) Fluorescent in situ hybridization for EGFP following DMSO or DAPT treatment. (H,I) Quantification of fluorescence detected following treatments in F and G. n=6 for each treatment. ***P<0.0001. Embryos were exposed to 10 or 100 µM DAPT, or DMSO, from 24 to 48 hpf (B,D) or from 24 to 72 hpf (C,E). Scale bars: 100µm.