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Fig. 3
EBD will be taken up by fibers with damaged membranes. (A) Wildtype siblings showing no EBD fluorescence in muscle fibers. (B) Sapje homozygous mutant with EBD fluorescence within multiple muscle fibers (arrows). All larvae were injected with the EBD injection mix and analyzed after a 4 hr incubation period at 3 dpf. Siblings and mutants were sorted by muscle fiber detachment prior to CCV injection.
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Full text @ J. Vis. Exp.