ZFIN Image: Nikolaou et al., 2015, Fig. S5

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Figure Caption

Fig. S5

Tectal proliferation and differentiation are unaffected in astray mutants (related to Figure 3). Measurement of proliferation and differentiation in WT (n = 4) and ast^ti272z (n = 5) larvae at 48 hpf. (A-B) Single confocal sections showing dorsal views of WT (A) and ast^ti272z (B) embryos immunostained for PH-3 and HuC/D to mark mitotic and postmitotic cells, respectively. TO-PRO-3 was also used to stain cell nuclei. Dashed line outlines the border of each tectal hemisphere. Scale bar represents 50 �m. A, anterior; L, lateral. (C-D) Quantification of the degree of proliferation and differentiation in the tectum. For the number of mitotic cells (PH-3⁺) (C) 12.6 � 1.4 for WT and 12.4 � 1.2 for ast^ti272z; for the number of post-mitotic cells (HuC/D⁺) (D) 119.1 � 7.4 for WT and 113.6 � 9.4 for ast^ti272z. All graphs show mean values � SEM. ns, not significant, unpaired t-test.

Acknowledgments

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