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Fig. 7
The short segment phenotype following Hsp47-MO treatment is due to formation of premature joints. ZNS5 immunostaining detects osteoblasts and joint-forming cells. Brightfield (left) and ZNS5 immunostaining (right) images are shown. ZNS5 positive joints (indicated by arrows) are observed in both standard control (top row) and targeting MO (bottom row) treated fins. Scale bar is 50µm and applies to all panels.
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Reprinted from Mechanisms of Development, 138 Pt 3, Bhadra, J., Iovine, M.K., Hsp47 mediates Cx43-dependent skeletal growth and patterning in the regenerating fin, 364-74, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.