ZFIN Image: Bhadra et al., 2015, Fig. 4

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Figure Caption

Fig. 4

Morpholino mediated knockdown of Hsp47 results in reduced expression in WT regenerating fins. (A) Longitudinal sections of WT 5 dpa fins following treatment with standard control or targeting MO against serpinh1b. Hsp47-MO treated fins show reduced level of Hsp47 expression. Hsp47 (red), nuclei are stained with the far red dye To-Pro (blue). ‘ble’ indicates basal layer of epithelium; ‘b’ blastema; ‘m’ mesenchyme, and ‘e’ epidermis. Scale bar is 10µm, and applies to all panels in A. (B) Immunoblots confirming reduced level of Hsp47 following Hsp47 knockdown. Hsp47 MO treated fins (MO) are compared to fins injected with standard control MO. Tubulin is used as loading control. (C) Bar graph depicting the ImageJ analysis of the ratio of Hsp47 levels between sof ^b123 or alf ^dty86 and wild-type (normalized by the tubulin loading control). Hsp47 protein levels are reduced in sof ^b123 and upregulated in alf ^dty86 relative to WT (represented as a relative density of 1).

Acknowledgments

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Reprinted from Mechanisms of Development, 138 Pt 3, Bhadra, J., Iovine, M.K., Hsp47 mediates Cx43-dependent skeletal growth and patterning in the regenerating fin, 364-74, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.