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Fig. S3
Lh3 modifies Collagens and is required for Col4a5 localization, related to figures 4 and 5
(A) Lh3 glycosylation sites are present in the three collagens that we tested for roles in motor axon regeneration (grey bars - schematic of protein; red stripes - putative lh3 modification sites).
(B) Tg(sox10:col4a5myc) is expressed diffusely throughout the cells in wild type siblings and associates with membranes, but in lh3 mutants
(C) Col4a5myc aggregates in large puncta in the cytoplasm (n > 10 embryos, 50 cells per condition; Scale bar = 5µm).
(D) col18a1 and col19a1 are not required to guide regenerating dorsal fascicles (n > 20 larvae, 200 nerves per condition).