ZFIN Image: Chua et al., 2015, Fig. 2

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Figure Caption

Fig. 2

Embryonic development and analysis of cell death in wild-type and mdm2^-/- embryos. Embryos were obtained from an incross of wild-type or mdm2^+/- zebrafish. (a) Brightfield images of wild-type and mdm2^-/-embryos at matching developmental stages show the lethal phenotype due to the homozygous loss of mdm2. (b) The 24-hpf wild-type and mdm2^-/- embryos were stained live with acridine orange and imaged under ultraviloet light. Increased green fluorescence in mdm2^-/- embryos compared with wild-type embryos suggests the presence of more apoptotic cells. (c) Whole-cell lysate was prepared from a number of wild-type/mdm2^+/- and mdm2^-/- embryos and used in a Caspase 3/7 assay. Cells from mdm2^-/- embryos have higher Caspase 3/7 activity compared with wild-type/mdm2^+/- siblings.

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Acknowledgments

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