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Fig. 4
Skin tumor formation in Tg(krt14: MEK2S219D-GFP) zebrafish. Skin cells proliferated and formed papilla from 3dfp (b, B′, white arrow) on the yolk skin in Tg(krt14: MEK2S219D-GFP) embryos to form a skin tumor at 6 dpf (c, and C′; lateral view, d and D′, white-dots area; ventral view, e and E′, white-dots area). Tg(krt14:GFP) embryos had developed normal skin cells at 6 dpf (a, A′). An immunohistochemical experiment was used to detect ERK phosphorylation in skin tumors. p-ERK was detected by a p-ERK monoclonal antibody and visualized by DAB (brown). Nuclei were counterstained with hematoxylin (blue) (f, F′)
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Full text @ J. Biomed. Sci.