ZFIN Image: Gokey et al., 2015, Fig. S7

Image

Figure Caption

Fig. S7 Atp6v1a localization in DFCs is altered in Atp6ap1b depleted embryos. (A-B) Confocal sections through a subset of DFCs labeled with an Atp6v1a antibody from Genescript. Punctate Atp6v1a signals were detected in the cytoplasm and along some plasma membranes (arrows) marked by Jup antibodies in DFCs in Tg(sox17:GFP) embryos injected with control MO (A). Plasma membrane association of Atpv1a signals was still detected (arrow), but was reduced in Atp6ap1b MO embryos (B). (C) The percentage of DFCs found to have Atp6v1a puncta associated with Jup staining at the plasma membrane. (D) Overall Atpv1a fluorescence in DFCs.

Acknowledgments

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Reprinted from Developmental Biology, 407(1), Gokey, J.J., Dasgupta, A., Amack, J.D., The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish, 115-30, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.