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Fig. S5 Concanamycin A treatments are effective in DFCs within 30 min. (A-B) The vital dye LysoTracker, which labels acidic organelles, was used to monitor efficacy of whole-embryo concanamycin A treatments. DFCs were labeled with GFP by sox17:GFP transgene expression. After 30 min, fluorescent LysoTracker signal was greatly reduced in live embryos treated with concanamycin (B) as compared to control embryos treated with DMSO (A).
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Reprinted from Developmental Biology, 407(1), Gokey, J.J., Dasgupta, A., Amack, J.D., The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish, 115-30, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.