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Fig. S2 disc1 expression is greatly reduced in iguts294e mutant embryos at 50 hpf. Whole mount in situ hybridization analysis for disc1 expression in iguts294e (B) revealed a complete loss of midline (arrow in B) and otic vesicle expression compared with sibling controls (A). Transverse sections at the level of rhombomeres 5/6 shown.
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