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Fig. S7
Related to Figure 7.
(A)-(B) Bright field images of eyes ofTg(HS-OS-Plk4) fish transplanted with wild type cells, showing big disproportion in size between the eyes. Scale bars are 100µm.
(C) Confocal scans of retinae of fish following transplantation of WT cells, expressing Ras-GFP (grey) and HS-H2B-RFP (green). Top and middle panels show Tg(HS-OS-Plk4) fish depicted in (A). Bottom panel shows a WT, transplanted fish (control). HS applied at 24hpf, fish were fixed 2.5 days later (control)/ 4 days later (in OS-Plk4 background to compensate for significant developmental abnormalities causing delay) and stained with DAPI (grey/magenta). In the HS-OS-Plk4 background WT cells repopulate the tissue and substantially restore neuronal lamination (top panel). Contralateral eye shows very few transplanted WT cells and displays significant tissue disorganization (middle panel). In control, distinct, separated clones of transplanted cells are present (bottom panel).
(D) Confocal scans of retinae of fish following transplantation of WT cells, expressing Ras-GFP (grey) and HS-H2B-RFP (green). Top and middle panels show Tg(HS-OS-Plk4) fish depicted in (B). Bottom panel shows a WT, transplanted fish (control). HS applied at 24hpf, fish were fixed 2.5 days later and stained with DAPI (grey/magenta). In the HS-OS-Plk4 background WT cells repopulate the tissue and substantially restore neuronal lamination (top panel). Contralateral eye shows very few transplanted WT cells and displays significant tissue disorganization (middle panel). In control, distinct, separated clones of transplanted cells are present (bottom panel).
All scale bars are 50µm unless stated otherwise.