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Fig. 7
WT Cells Can Compensate for Apoptotic Cell Loss Resulting from Centriole Amplification
(A and B) Confocal scans of the retina of a WT fish (A) and a fish injected with HS-OS-Plk4 and HS-H2B-GFP constructs (green) (B); HS was applied at 24 hpf, and these embryos were fixed at 80 hpf and stained for phospho-histone 3 (pH3) (mitotic cells, magenta) and DAPI (nuclei, blue). Arrows point at mitotic cells at ectopic positions.
(C) Bright-field images of the eyes of Tg(HS-OS-Plk4) fish transplanted with WT cells. Scale bar, 100 µm.
(D) Confocal scans of the retinae of fish following transplantation of WT cells, expressing Ras-GFP (gray) and HS-H2B-RFP (green). Left and middle panels show Tg(HS-OS-Plk4) fish depicted in (C). Right panel shows a WT transplanted fish (control); HS was applied at 24 hpf, and these embryos were fixed 2.5 days later (control)/4 days later in OS-Plk4 background to compensate for developmental delay and stained with DAPI (gray/magenta).
Scale bars, 50 µm unless otherwise stated.
See also Figure S7.