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Fig. S2
mfap4 transgenes are robustly expressed in adult zebrafish macrophages.
a. Frozen 20 µm section of 3 month old adult Tg(mfap4:tdTomato-CAAX)xt6 zebrafish liver tissue, 2 weeks post-infection (wpi) with approximately 400 c.f.u. of M. marinum expressing the Cerulean (cyan) fluorescent protein. i. Macrophages expressing tdTomato. The stellate morphology expected of Kupffer cells, specialized resident macrophages of the liver, can be seen. ii. M. marinum. iii. DAPI. iv. Merge of iiii. b. Frozen 20 µm section of kidney tissue from the same fish. i. Macrophages expressing tdTomato. ii. M. marinum. iii. DAPI. iv. Merge of i-iii. For both a and b, tdTomato (macrophage) and Cerulean (M. marinum) fluorescence signals are derived directly from fixed fluorescent protein; other than DAPI, no additional staining was performed. Scale bars = 50 µm.
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