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Fig. S7 Rdh10a expression in heat-shocked Tg(hsp70/:pax2a) embryos.
(A, B) Rdh10a expression (blue) at 24 hpf in heat-shocked control sibling non-transgenic and hemizygous Tg(hsp70l:pax2a) embryos. ISH for pax2a (red) was performed to distinguish between control sibling and transgene carriers. 12 of the 27 embryos did not have ectopic pax2a expression indicating they were non-trangenic, while 15 of the 27 embryos has ectopic pax2a expression indicating they were the Tg(hsp70l:pax2a) embryos. There was no discernible difference between rdh10a expression in the eyes (arrows) of control embryos and embryos with increased Pax2a.