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Fig. S5
tert silencing in the injured hearts inhibits the proliferative response of cardiac cells.
(A) tert activity assay in stab-injured hearts cultured for 3 days in the presence of 10 µM gfp vivo Morpholino or tert vivo Morpholino. Data are means ± SD from 2 pools of 3 hearts per condition (p<0.05, unpaired Student’s t-test). A second experiment using 2 additional control and tert vivoMO replicates at 15 µM (3 pooled hearts per replicate) yielded the same result (data not shown).
(B) Immunfluorescence analysis of gfp and tert vivoMO-treated hearts. Stab-injured hearts were immunostained for PCNA (green) and myosin heavy chain (MHC, red), and nuclei were counterstained with DAPI (blue). Boxed areas are shown at higher magnification in the lower panels. Bars, 200 µm (whole heart images), 50 µm (zoomed views).
(C) Quantification of the percentage of PCNA-positive cells per total cell nuclei in hearts treated with gfp or tert vivoMO. Data are means ± SD from 4-5 hearts per condition (p<0.05, unpaired Student’s t-test).
Related to Figure 3.