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Fig. S4
Clone size increases in a zone extending up to 250µm from the wound centre.
A-B RasG12VeGFP larvae were left unwounded (A) or laser wounded (B) at 2dpf just dorsal to the urogenital opening. Larvae were left to grow for 3 days before being fixed and analysed for pre-neoplastic cell number (n=30 larvae in each group). (A′′ and B′′) show the analysis performed to measure the distance from a clone to the centre of the wound. (A′′′ and B′′′) show the boundaries of, 50-150µm, 150-250µm and >250 µm which were used to define clone location, as plotted in (C). The approximate domain for imaging is indicated by a box in the schematic.
Scale bars represent 50µm.