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Fig. S3
Timecourse of leukocyte recruitment to adult fin wounds.
A-F Adult zebrafish were analysed for inflammatory cell recruitment by resecting a small portion of the caudal tail fin. Three 6 month old LysC:dsRed; mpeg:FRET fish were fixed each day post wounding and whole-mount immuno-stained for LysC+ neutrophils (red) and mpeg+ macrophages (green) for seven days post wounding.
G-G′′′ A zoom in of immune cell recruitment at two days post wound (C) is shown in panel.
H- Neutrophil and macrophage recruitment is quantified, calculated by the number of particles counted automatically in ImageJ.
Scale bars represent 150µm (A-F and J-O) and 50µm (G).
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Full text @ EMBO J.