ZFIN Image: Antonio et al., 2015, Fig. S1

Image

Figure Caption

Fig. S1

Standard larval model for studying immune cell:pre-neoplastic cell interactions.

A. Schematic to illustrate the larval model, whereby oncogenic Human RasG12V is used to drive cell transformation in a tissue specific manner, in melanocytes and mucus-secreting goblet cells by a kit-a enhancer trap (ET30).

B. DIC microscopy of a 5dpf kita:RasG12VeGFP zebrafish larvae.

C. Confocal laser scanning microscopy image of a 5dpf kita:RasG12VeGFP; LysC:dsRed zebrafish larvae. (A′, B′, C′) Zoom in of the area around the cloaca to illustrate the region frequently imaged.

D. and E Adult fish occasionally develop large pigmented tumours, particularly at exposed sites such as the ventral fin by the cloaca or tail.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO J.